Fig 1: Comparison of the functional characteristics of endothelial cells in hiPSC-ECs and HCMECs. The triplicate tube formation experiment was carried out. Representative images of tube formation are shown (A,B). The scale bar was 200 µm. (C) Tube length of formed tubes in hiPSC-ECs and HCMECs. (D) ET-1 levels in response to phenylephrine (PE) were measured using the Elisa method in hiPSC-ECs and HCMECs. n = 4, * p < 0.05; ** p < 0.005. (E) NO release was measured in hiPSC-ECs and HCMECs treated with acetylcholine (Ach). n = 4, * p < 0.05. (F,G) Fluorescence staining showing the degree of LDL uptake in hiPSC-ECs and HCMECs. Scale bar was 100 µm. (H) Relative fluorescence intensity of LDL in hiPSC-ECs and HCMECs. All data shown are mean ± SEM. n = 3, * p < 0.05; ** p < 0.005.
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